Services
The Ralph M. Parsons Plant Transformation Facility at UC Davis provides routine transformation services for the following species. If you require pricing for a species not listed below, please contact the facility at 530 752-3766 or e-mail David Tricoli.
The facility has limited molecular capacity. Therefore, clients within the state of California are required to provide the facility with an engineered Agrobacterium culture containing their gene of interest. Clients outside of the state of California should submit plasmid DNA along with the appropriate primers to verify that we have successfully transformed Agrobacterium with your plasmid.
Transformation Services Pricing Schedule
| Alfalfa – Medicago sativa | ||
|---|---|---|
| Cultivar | Selection System | Cost* |
| Regen SY, Regen S | Kanamycin | 10 hrs @ $50/hr = $500 |
| Canola – Brassica napus | ||
| Cultivar | Selection System | Cost* |
| Westar | Kanamycin | 10 hrs @ $50/hr = $500 |
| Citrus – Poncirus trifoliata (L.) Raf. x C. sinensis | ||
| Cultivar | Selection System | Cost* |
| Carizzo | Kanamycin | 10 hrs @ $50/hr = $500 |
| Carrot – Daucus carota | ||
| Cultivar | Selection System | Cost* |
| Imperator | Kanamycin | 10 hrs @ $50/hr = $500 |
| Grape – Vitis vinifera | ||
| Cultivar | Selection System | Cost* |
| Thompson Seedless | Kanamycin, Hygromycin | 20 hrs @ $50/hr = $1,000 |
| Lettuce – Lactuca sativa | ||
| Cultivar | Selection System | Cost* |
| Most cultivars | Kanamycin | 10 hrs @ $50/hr = $500 |
| Rice – Oryza sativa | ||
| Cultivar | Selection System | Cost* |
| Kitaake, Nipponbare | Hygromycin | 10 hrs @ $50/hr = $500 |
| Tobacco – Nicotiana benthamiana | ||
| Cultivar | Selection System | Cost* |
| Kanamycin, Hygromycin, Glufosinate | 10 hrs @ $50/hr = $500 | |
| Tobacco – Nicotiana tabacum | ||
| Cultivar | Selection System | Cost* |
| Sansum NN, SR-1, Xanthi | Kanamycin, Hygromycin, Glufosinate | 5 hrs @ $50/hr = $250 |
| Tomato – Lycopersicom esculentum | ||
| Cultivar | Selection System | Cost* |
| Moneymaker, T-5, UC-82B, VF36, MicroTom, etc** | Kanamycin, Hygromycin, Glufosinate | 10 hrs @ $50/hr = $500 |
| Walnut – Juglans regia | ||
| Cultivar | Selection System | Cost* |
| CR-1 | Kanamycin | 30 hrs @ $50/hr = $1,500 |
| Walnut – Juglans hindsii x J. regia | ||
| Cultivar | Selection System | Cost* |
| PX-1 | Kanamycin | 30 hrs @ $50/hr = $1,500 |
* Cost = Number of hours required to construct 10 independent transgenic events @ $50/hr.
** Many other cultivars can be transformed, contact the Facility for details.
Additional Species
We also have transformation experience for the following crop species: apple, broccoli, cucumber, eggplant, Ipomoea, melon, Mimulus and squash. Please contact us for more information and pricing.
Minimum Fee
We make every effort to successfully produce and deliver the number of plants requested by clients using our transformation service. However, on occasion there are extenuating circumstances that prevent us from completing an order. These include; defective or contaminated Agrobacterium cultures, constructs that inadvertently lack a selectable marker gene, constructs that contain genes of interest that are lethal to plant tissue, and constructs that contain genes that act as phytohormones thereby interfering with the normal plant regeneration process. Since the nature of our work requires that we invest a significant amount of up front effort prior to identifying these types of problems, the customer is required to pay 20% of the total recharge fee even if we are unsuccessful in regenerating the requested number of plants.
Protocol Development
The Facility’s staff has extensive experience in the development of transformation protocols for species for which transformation methodologies are not available or are currently inefficient. The facility’s recharge rate for protocol development is $50/hour. Please contact us at 530 752-3766 or e-mail David Tricoli for more information.